With regarding to FISH and immunohistochemical study, we had demonstrated that PBSCs was observed on hair follicle characterized by increasing collagen fiber, elastin fiber and hyaluronic acid as well as its receptor CD44 on dermis and subcutaneous after injection of GCSF-mobilized PBSCs. These will contribute skin more tightly and elasticity. In terms of epidermis, by semiquantity of involucin and integrade ratio using immnohistochemical stain, we demonstrated PBSC might increase epidermis cell renewal accompanied by reducing cornified and spinous layer as well as basal layer pigmentation.

In summary, these consequences might lead to restore aging skin from 90 months approaching into 5 months of juvenile skin.

We concluded that PBSCs could help the hair follicle secreted homing factors to recruit stem cell and produced a beneficial environment for aging skin rejuvenation.