Why store peripheral stem cells?

Aging is inevitable. As we age, our cells are subject to external and internal aging factors, making our DNA susceptible to damage and mutation and causing our cell’s function and ability to deteriorate.

We have about 60 trillion cells in our human body. Everyday, around 600 to 1200 billion cells die in our body. At the same time, adult stem cells serve as the maintenance and repair system to replenish damaged cells. When the rate of regeneration cannot match the rate of cell death, we age and diseases start to surface. Ongoing cellular damage reduces regenerative potential. The damaged cells would not perform as well as younger, undamaged cells.

Therefore, it is necessary to store them when you are still healthy. Cryogenic storage can ensure that your stem cells stay in the prime state available for future therapeutic application. As cell therapy is the future of Regenerative Medicine, your stored cells allow you to take advantage of all the developments taking place today.

Can I use my child’s umbilical cord blood as a source of younger stem cells?

Umbilical cord blood must be collected immediately upon birth. While there are hematopoietic stem cells and mesenchymal stem cells in the cord blood, the numbers thereof are limited. Furthermore, for average adult to make practical use of the cord blood stem cells, there must be sufficient amount of the stem cells. If this is the case, pairing is required. Often, the pairing process fails due to issues of repellence. If not for several technical and procedural difficulties, cord blood stem cells are a fantastic source for stem cells.

Although most people have missed this prime window of opportunity to bank stem cells, one can still collect peripheral blood stem cells. By collecting peripheral blood bone marrow stem cells, we not only collect hematopoietic stem cells, but also mesenchymal stem cells, VSEL, progenitor cells, and immunocyte cells. So, for the average adults who are no longer capable to collect stem cells, we provide a great way to collect healthy stem cells in sufficient amounts for future therapeutic use.

What is G-CSF?

G-CSF (Granulocyte colony Stimulating Factor) is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Beside the effect on the hematopoietic system, G-CSF can also act on neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis.

G-CSF is also used to increase the number of hematopoietic stem cells before collection, which has been the method used at major medical research centers for HSCs transplantation.

Why inject G-CSF?

After the injection of G-CSF, several different cells, including but not limited to, HSCs, MSCs, B-cell, T-Cell Lymphoma, will be stimulated and released into the bloodstream. The amount of stem cells stimulated and released into the bloodstream will vary depending on the conditions of each individual. It is possible for the amount to increase ten- or even hundredfold. Using the technique for collecting peripheral blood bone marrow stem cells, we are able to collect these cells at one time.

Is G-CSF safe?

In the beginning of 2011, the Journal of Blood Society published a paper on the safety of G-CSF administration. The number of subjects who participated in the experiments neared 4000. After 12 years follow up, the subjects displayed no side effects and adverse event. The article was published in January, 2011.

Another G-CSF follow up study is conducted in 2013. According to the results, the vast majority of PBSC donors consider their general condition excellent or good throughout the observation period.

For reference, please refer to: http://bloodjournal.hematologylibrary. & https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776391/

Besides infectious diseases and coagulant diseases, what other conditions or circumstances would make an individual inapt to collect peripheral blood stem cells?

■ Individuals with chronic Myelogenous Leukemia or Aplastic Amenia, or individuals undergoing chemotherapy.

■ Pregnant women, and women in the process of breast-milk feeding.

■ Individuals with Deep Vein Thrombosis or Pulmonary Thrombo-Embolic disease.

■ Individuals with mental diseases or individuals on drugs containing Lithium.

■ Individuals who take antibiotics to reduce inflammations.

If an individual is under any of the conditions or circumstances listed above, then further steps must be taken and the process of collecting peripheral blood stem cells must be delayed.

What is the age limit for peripheral blood stem cells collection?

As long as you are above twenty years old and maintain a rather healthy condition, you are eligible to have your peripheral blood stem cells collected. There is no upper age limit for the collection. However, as you age, both the number of your stem cells and the quality (repopulating ability) of which may decrease. As a result, we advise that you put in reserve your stem cells as early as possible. 

What’s the best interval between multiple PBSC collection?

According to clinical trials and research conducted, as early as 22 days after the first collection may you undergo another collection. The common standard in the U.S. today is to wait three months for the next collection. To ensure efficient collection, we would recommend next harvesting must begin when your physical and mental states restore to their ideal levels. Thus, at least one year after prior mobilization is recommended.

Could I collect my peripheral blood stem cells when I’m sick?

For certain diseases or illnesses, the individual could still collect his or her stem cells after proper treatment. However, ailments or medications may have affected stem cells counts. It is possible that such negative impact may cause the body to response poorly to G-CSF injection. If poor mobilization happens, the number of stem cells collected are not adequate for practical use. Therefore, it is better to preserve your stem cells when you’re young and healthy.

Where does Enhance Cell Bank store my stem cells?

We store your stem cells in our state-of-the-art processing and storage lab. We use a class 100,000 clean room to minimize viable and nonviable particle counts. Designed for efficiency and sterility, the lab equipped with the same freezers used by major research institutions. The quality management system also achieved ISO 9001, showing our commitment to our quality and service.

Why store stem cells in multiple compartments?

In order to preserve the flexibility for different therapeutic use, we would sort your stem cells, depending on the number and density thereof, into cryo tubes and reserve them accordingly.

How would Enhance Cells Bank perform the quality control in order to eliminate any foreseeable risks?

Our lab specialists inspect and monitor every step of the process with discretion.

The entire process follows high GTP (Good Tissue Practice) Laboratory standards in a dust-free environment. To ensure the purity and safety of the stem cells, it’s necessary to design and implement a comprehensive testing program. Sterility testing ensures that the cells are free from bacteria, fungi, and mold, which can be introduced through contaminated operators, or contaminated facilities. We’ll notify you as soon as your detailed report that lists microbe inspection result, accurate count of the number of stem cells and the location at which the stem cells are stored. Annual viability tests will perform to ensure that the frozen cells maintaining high cell viability.

How long can stem cells be stored ?

Once processing is complete, our lab specialists transfer your stem cells to cryo tubes for cryopreservation. The cryo tubes are then frozen and stored at or below -180 degrees Celsius, where biological activity ceases. Scientists believe that if stem cells are properly stored and processed, they should last indefinitely. Therefore, your stem cells remain viable for therapeutic use when thawed.

How does Enhance Cell Bank preserve the vitality of stem cells?

A key to preserve both viability and functionality of stem cells requires very dedicated efforts. Enhance Cell Bank specifically developed a rapid and efficient process to eliminate any potential damage to stem cells. This process results in highest stem cells survival rate. A temperature-controlled cryopreservation instrument is used to freeze stem cells to prevent any ice crystal formation during the freezing process. Subsequently, the frozen stem cells are transferred to a gas-phase liquid nitrogen tank which maintains at -180C. The liquid nitrogen tanks are monitored 24 hours daily. All liquid nitrogen tanks are connected to 11-tons filler liquid nitrogen tanks and are automatically replenished with liquid nitrogen controlled by computer to ensure a proper low temperature environment. A 3Q temperature calibration of those liquid tanks is conducted annually to comply with the Regulation of Department of Health such that all liquid nitrogen tanks have the highest level of temperature stability.